Week10ReviewSheet I Exercise1-Disinfectants 1. Whatdoesbactericidalmean?Bacteriostatic?Virucidal?Fungistatic? 2. Whyarecontrolculturesnecessaryinevaluatingdisinfectants? 3. Whatfactorscaninfluencetheactivityofadisinfectant? 4. Whydomicroorganismsdifferintheirresponsetodisinfectants? 5. Whatmicroorganismsaremostsusceptibleto disinfectants? EXERCISE2-AntimicrobialAgentSusceptibilityTestingand Resistance 1. Whatismeantbyantimicrobialresistance?Susceptibility? 2. Whyarepureculturesusedforantimicrobialsusceptibilitytesting? 3. Woulditbeacceptabletouseamixedcultureforthistest?Why? 4. Listthreefactorsthatcaninfluencetheaccuracyofthetest. 5. Whenperformingabrothdilutiontest,whyis itnecessaryto includea growthcontroltube? Asterilitycontroltube? EXERCISE3-TheEnterobacteriaceae(EntericBacilli) 1. Whatdoestheterm IMViCmean? 2. Whyis theIMViCusefulinidentifyingEnterobacteriaceae?Arefurtherbiochemicaltests necessaryforcompleteidentification? 3. WhatdiagnostictestdifferentiatesProteusandProvidenciaspeciesfrom otherEnterobacteriaceae? 4. HowisE.colidistinguishedfrom P. vulgarisonMacConkeyagar?OnaTSIslant? 5. Whyisitimportanttodifferentiateglucosenonfermentersfrom Enterobacteriaceae? EXERCISE4–Streptococci,Pneumococci,and Enterococci 1. Differentiatethemicroscopicmorphologyofstreptococciandpneumococciasseenby Gramstain. 2. Whattypeof hemolysisis producedbyS. pneumoniae? 3. HowisS.pneumoniaedistinguishedfrom otherstreptococciwiththesamehemolytic properties? 4. Describethehemolysisproducedbyalpha-hemolytic,beta-hemolytic,andnonhemolytic streptococci. 5. Whattypeofhemolysisis displayedbythegroupablestreptococcithataremostpathogenic forhumanbeings? EXERCISE5-UrineCultureTechniques 1. Whatis bacteriuria?Whenis itsignificant? 2. Howdomicroorganismsentertheurinarytract? 3. Whyisasepticurinecollectionimportantwhenculturesareordered? 4. Listfivebacteriathatcancauseurinarytractinfection. 5. Ifyoucounted20 coloniesfrom a0.01-mlinoculumofa1:10dilutionofurine,howmany organismspermilliliterof specimenwouldyoureport?Isthisnumbersignificant? Week10ReviewSheet II EXERCISE1-SerodiagnosisofInfectiousDisease 1. Defineserumtiter. 2. Whatareacuteandconvalescentsera?Whymustbothbetestedtomakeaserological diagnosisof infectiousdisease? 3. Whatis thedifferencebetweenanagglutinationtestanda precipitationtest? 4. Ina pairedserumsample,whattestresultsindicaterecentinfection? 5. Whatis ahumoralantibody? EXERCISE2-CulturingMicroorganismsfromtheEnvironment 1. Whydomicrobiologistswearlaboratorycoats?Didyouconfirmthatthisisnecessary? 2. Whyisitnecessarytowearclean,protectiveclothingwhencaringforapatient? 3. Whyshouldhairbekeptcleanandoutofthewaywhencaringforpatients? 4. Howcanthenumberofmicroorganismsintheenvironmentbecontrolled? 5. Whenandwhyis handwashingimportantinpatientcare? 6. Howcanthosewhocareforpatientsavoidspreadingmicroorganismsamongthem? EXERCISE3-Staphylococci 1. DifferentiatethemicroscopicmorphologyofstaphylococciandstreptococciasseenbyGramstain. 2. Whatarethetwotypesofstaphylococcalcoagulase? 3. Whatis proteinA?Describeonemethodofdetectingit. 4. Whatpropertiesof S.aureusdistinguishitfrom S.epidermidisandS.saprophyticus? 5. Whyarestaphylococcalinfectionsfrequentamonghospitalpatients? __________________________________________________________________________ Must have APA citation and include questions and answers.
Week10ReviewSheet I Exercise1-Disinfectants 1.  Whatdoesbactericidalmean?Bacteriostatic?Virucidal?Fungistatic? 2.  Whyarecontrolculturesnecessaryinevaluatingdisinfectants? 3.  Whatfactorscaninfluencetheactivityofadisinfectant? 4.  Whydomicroorganismsdifferintheirresponsetodisinfectants? 5.  Whatmicroorganismsaremostsusceptibleto disinfectants? EXERCISE2-AntimicrobialAgentSusceptibilityTestingand Resistance 1.  Whatismeantbyantimicrobialresistance?Susceptibility? 2.  Whyarepureculturesusedforantimicrobialsusceptibilitytesting? 3.  Woulditbeacceptabletouseamixedcultureforthistest?Why? 4.  Listthreefactorsthatcaninfluencetheaccuracyofthetest. 5.  Whenperformingabrothdilutiontest,whyis itnecessaryto…
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